Nanofibrous Gelatin-Based Biomaterial with Improved Biomimicry Using D-Periodic Self-Assembled Atelocollagen

Design of bioinspired materials that mimic the extracellular matrix (ECM) at nanoscale is a challenge in tissue engineering. While nanofibrillar gelatin chemical composition and nano-architecture natural ECM collagen components, it lacks characteristic D-staggered array (D-periodicity) 67 nm, which an important cue terms cell recognition adhesion properties. In this study, nanofibrous with improved biomimicry achieved using formulation including minimal content D-periodic self-assembled atelocollagen. We suggest processing route approach consisting thermally induced phase separation based biopolymeric mixture precursor followed by chemical-free material cross-linking. The nanostructure characterized field emission gun scanning electron microscopy (FEG-SEM), transmission (TEM), wide angle X-ray diffraction (XRD) Fourier-transform infrared spectroscopy (FT-IR). culture assays indicate incorporation 2.6 wt.% atelocollagen to material, produces significant increase MC3T3-E1 mouse preosteoblast cells attachment human mesenchymal stem (hMSCs) proliferation, comparison related bare matrices. presented results demonstrate achievement efficient produce cost-effective, compositionally defined low immunogenic “collagen-like” instructive biomaterial, on gelatin.